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Objective To investigate the effects of gross saponins from Tribulus terrestris L (GSTT) on myocardial apoptosis, and its related gene bcl-2 and bax expression after hypoxia/reoxygenation in cultured myocardial cells of neonatal rat. Methods After myocardial hypoxia/ reoxygenation model was established by culturing primary myocardial cell of neonatal rat in vivo. The myocardial cells were divided into four groups, the sham-operated group, model group, large- and low-dose of GSTT groups. The apoptotic rate of myocardial cells was determined by flow cytometry, and the expression of bcl-2 and bax was detected by using immuno-histochemical method. Results Both large- and low- dose of GSTT could significantly decrease the apoptotic rate (P
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Objective To investigate the effect of GSTT on the release of inflammatory molecules such as TNF-? and IL-1? in rats after ischemia/reperfusion injury. Methods An in vivo model of myocardial ischemia 45min followed by 2h reperfusion was made by reversibly ligating coronary left descending artery. 32 Sprague Dawley (SD) rats were divided randomly to 4 groups, ie: sham-operated group, model group, large and dosage of GSTT. TNF-? and IL-1? concentrations were examined by radioimmunoassay. Results Compared with model group, large dosage of GSTT significantly decreased the contents of TNF-? and IL-1?(P
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Objective To investigate the preventive effects of total saponin from Tribulus terrestris (GSTT) on cardiomyocytes subjected to low oxygen tension and re-oxygenation injury in vitro. Methods First passage of cultured cardiomyocytes was subjected to hypoxia followed by oxygenatim. The cells were divided into 4 groups, namely normal group, injury group, and large and low dose of GSTT groups. The frequency of contraction, survival rate and release of myocardial enzymes were determined. The morphologic changes were observed with light and electron microscopy. Results Both large and low dose of GSTT significantly improved the survival rate of cardiomyocytes after hypoxia and re-oxygenation injury (P
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Objective To investigate the effects of gross saponin from Tribulus terrestris (GSTT) on myocardial ischemia/reperfusion (MI/R) injury. Methods A model of myocardial MI/R injury of the myocardium was reproduced by ligation of left descending artery for 45min followed by releasing the ligation for 2 hours in rats. 64 SD rats were divided randomly to 4 groups, i.e. sham-operated group, model group, large and low dose of GSTT groups. HE staining was examined to assess myocardial pathological changes. TTC staining was used to determine myocardial infarction area. Serum contents of lactate dehydrogenase (LDH), creatine phosphokinase (CPK) and malondialdehyde (MDA) were also determined. Results Both large and low dose of GSTT improved myocardial pathological changes after ischemia/reperfusion. Large dose of GSTT reduced myocardial infarction area (P
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Objective To investigate the effects of GSTT on expression of inflammatory molecule ICAM-1 and the infiltration of neutrophils in rats after myocardial ischemia/reperfusion (MI/R) injury. Methods A model of myocardial ischemia 45min followed by 2h reperfusion was made by reversibly ligating coronary left descending artery. 32 Sprague Dawley (SD) rats were divided randomly to 4 groups, ie: sham-operated group, model group, large and dosage of GSTT. HE staining was used to observe infiltrating neutophils. The activity of MPO was measured, too. Immunohistochemistry was used to determine ICAM-1 expression in myocardium. Results Light microscopy showed that the infiltration of neutrophils in both large and low dosage of GSTT group was less serious than that in model group. Both large and low dosage of GSTT significantly decreased MPO activity (P